Inactivation of Toluene 2-Monooxygenase in Burkholderia cepacia G4 by Alkynes
نویسندگان
چکیده
منابع مشابه
Inactivation of toluene 2-monooxygenase in Burkholderia cepacia G4 by alkynes.
High concentrations of acetylene (10 to 50% [vol/vol] gas phase) were required to inhibit the growth of Burkholderia cepacia G4 on toluene, while 1% (vol/vol) (gas phase) propyne or 1-butyne completely inhibited growth. Low concentrations of longer-chain alkynes (C5 to C10) were also effective inhibitors of toluene-dependent growth, and 2- and 3-alkynes were more potent inhibitors than their 1-...
متن کاملDegradation of Toluene and Trichloroethylene by Burkholderia cepacia G4 in Growth-Limited Fed-Batch Culture.
Burkholderia (Pseudomonas) cepacia G4 was cultivated in a fed-batch bioreactor on either toluene or toluene plus trichloroethylene (TCE). The culture was allowed to reach a constant cell density under conditions in which the amount of toluene supplied equals the maintenance energy demand of the culture. Compared with toluene only, the presence of TCE at a toluene/TCE ratio of 2.3 caused a fourf...
متن کاملSaturation mutagenesis of toluene ortho-monooxygenase of Burkholderia cepacia G4 for Enhanced 1-naphthol synthesis and chloroform degradation.
Directed evolution of toluene ortho-monooxygenase (TOM) of Burkholderia cepacia G4 previously created the hydroxylase alpha-subunit (TomA3) V106A variant (TOM-Green) with increased activity for both trichloroethylene degradation (twofold enhancement) and naphthalene oxidation (six-times-higher activity). In the present study, saturation mutagenesis was performed at position A106 with Escherichi...
متن کاملCometabolic Degradation Kinetics of Trichloroethylene Based on Toluene Enhancement by Encapsulated Burkholderia cepacia G4
The ability of encapsulated Burkholderia cepacia G4 (ATCC 53617) for trichloroethylene (TCE) degradation (1.5, 5, 10, and 20mg/L) in the presence of toluene (10 and 60mg/L) as enhancement substrate was evaluated experimentally. Burkholderia cepacia G4 cultures were encapsulated in cylindrical pellets (4mm in diameter and 4mm in height (preferred)) using polyethylene glycol (PEG). Higher transfo...
متن کاملCytotoxicity associated with trichloroethylene oxidation in Burkholderia cepacia G4.
The effects of trichloroethylene (TCE) oxidation on toluene 2-monooxygenase activity, general respiratory activity, and cell culturability were examined in the toluene-oxidizing bacterium Burkholderia cepacia G4. Nonspecific damage outpaced inactivation of toluene 2-monooxygenase in B. cepacia G4 cells. Cells that had degraded approximately 0.5 micromol of TCE (mg of cells(-1)) lost 95% of thei...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1999
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.65.2.632-639.1999